On August 6, 2019, JSR Life Sciences and its Chinese general agent,Gen-Hill Technology, held a technical exchange meeting for Bio-Thera Solutions, Ltd in Guangzhou.

Agenda of the Conference

 

Time：August 6th ,2019

Add：No. 155 Yaotianhe Street, Yonghe Economic Development Zone，Huangpu District, Guangzhou（Bio-Thera Solutions, Ltd）

President：Xiang Han

Conference Agenda：

1.Design Concept of Amsphere? A3

2.Performance Data

3.DBC & Impurity clearance (HCP, PrA leaching)

4.Alkaline stability

5.Fc-Fusion Protein

6.Evaluation Example at KBI

7.Life Cycle Study

8.Chromatography Scale Up

9.JSR Customer Support

 

Introduction of Protein A Affinity Filler



Protein A affinity chromatography packing is widely used for affinity purification of monoclonal antibodies or Fc fusion proteins because of its high selectivity. Due to the different elution performance, the affinity purification process of Protein A needs more development work, and has a close relationship with downstream process development. JSR Life Science has carried out a series of research and development work on this affinity chromatography filler, and introduced Amsphere TM A3, a very high quality filler.

Protein A has the ability to bind to antibodies without binding sites to antigens. Protein A can be specifically bound to the Fc segment of the antibody IgG molecule, and Protein A can be coupled to agarose gel as a ligand. When the samples such as antiserum and cell supernatant flow through it, the specific Fc segment of IgG will be bound to the ligand, and other proteins will flow through and after cleaning. The required IgG with high purity can be recovered by elution with corresponding buffers.

However, when the purified antibody is different, the binding capacity of different host antibodies is different. We also need to treat it according to the specific circumstances. We recommend the mAb IgG2a, IgG2b, IgG3, rabbit, human and pig polyclonal antibody to be purified by Protein A. Protein A affinity chromatography has become a widely used method for antibody purification. It can isolate and purify various mammalian antibodies of different subtypes or recombinant proteins containing Fc fragments from ascites, serum and cell culture supernatants or cell extracts.


Protein A Characteristics


1. Excellent load (DBC)

Compared with standard products in the market, DBC has increased by 20%-50%.

2. Impurity Removal Level of Industrial Standards

Removal of HCP, polymers, DNA and viruses reaches the level of market standard products.

3. Excellent alkali resistance stability

It can go through 100 cycles, each cycle alkali washing time 15 minutes, 0.1M NaOH CIP, maintain more than 90% of the load (DBC)

4. Good pressure-flow characteristics

400 cm/hr, < 3 bar (20 cm column height, 30 cm column diameter)

5. Attractive Economy

Because of its high yield and affordable price, the cost of protein A filler can be reduced by at least 50% compared with standard products in the market.